Experimental study on incidence of anti-CD36 antibodies in puerperae and platelet apoptosis induced by anti-CD36 antibodies

ZHOU Yan; ZHONG Zhoulin; LU Fang; LIU Jinlian; LI Lilan; WU Guoguang

doi:10.13201/j.issn.1004-2806.2021.08.001

Article navigation > J Clin Hematol > 2021 > 34(8): 531-535

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ZHOU Yan, ZHONG Zhoulin, LU Fang, et al. Experimental study on incidence of anti-CD36 antibodies in puerperae and platelet apoptosis induced by anti-CD36 antibodies[J]. J Clin Hematol, 2021, 34(8): 531-535. doi: 10.13201/j.issn.1004-2806.2021.08.001

Citation:

Experimental study on incidence of anti-CD36 antibodies in puerperae and platelet apoptosis induced by anti-CD36 antibodies

Nanning Institute of Transfusion Medicine, Nanning, 530007, China

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Corresponding author: WU Guoguang, E-mail: guangwu@szonline.net

Received Date: 11 January 2021

Abstract

Abstract

Objective: To detect and analyze the incidence of anti-CD36 antibodies produced by puerperae in Nan-ning, and investigate the effect of anti-CD36 antibody on platelet apoptosis.Methods: Anti-CD36 antibodies were detected and identified by flow cytometry(FCM) and fast monoclonal antibody-specific immobilization of platelet antigens(F-MAIPA) from 303 puerpera cases in Nanning. The CD36 antigen expression on platelets and monocytes of the puerperae who contained anti-CD36 antibodies were detected by FCM; platelet apoptosis induced by anti-CD36 antibodies were analyzed by FCM and ELISA; the platelet count, clinical manifestation and the treatment of the newborns were monitored.Results: Among the 303 puerpera cases, 11 cases who were typeⅠCD36 deficiency were detected anti-CD36 antibodies in their sera, the incidence of anti-CD36 antibody was 3.63%. 2 sera(case 6 and 8) in the 11 cases contained anti-CD36 antibodies could induce platelet apoptosis. All the newborns delivered by 11 cases presented with thrombocytopenia and needle-like subcutaneous haemorrhage, but no intracranial hemorrhage or other visceral hemorrhage. After intravenous immunoglobulin/steroid therapy, platelet count of these newborns was increased to the normal range.Conclusion: The occurrence of anti-CD36 antibody from puerperae in Nanning is mainly in Zhuang population. The 11 newborns are approved to be neonatal alloimmune thrombocytopenia caused by anti-CD36 antibodies. Some anti-CD36 antibodies could induce platelet apoptosis, and its structural characteristics need to be studied.
- anti-CD36 antibody /
- puerpera /
- platelet apoptosis

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References

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